Human FGF-acidic/FGF1 Recombinant Protein #27398
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 16.0 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-acidic. |
| Activity | The bioactivity of recombinant human FGF-acidic was determined in a NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 2 ng/mL. |
Source / Purification
Recombinant human FGF-acidic was expressed in E. coli and is supplied in a lyophilized form.
Background
FGF-acidic (FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF-acidic is involved in wound repair, angiogenesis, and development (1). FGF-acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF-acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF-acidic binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).
Limited Uses
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