|Purity||A greater than or equal to 95% purity was determined by SDS-PAGE.|
|Endotoxin||Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-acidic.|
|Activity||The bioactivity of recombinant human FGF-acidic was determined in a NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 2 ng/mL.|
Recombinant human FGF-acidic was expressed in E. coli and is supplied in a lyophilized form.
FGF-acidic (FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF-acidic is involved in wound repair, angiogenesis, and development (1). FGF-acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF-acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF-acidic binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).
UniProt ID: P05230
Entrez-Gene Id: 2246
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