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Product last modified at: 2024-06-05T16:15:09.108Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human FGF-basic/FGF2 (147 aa) Recombinant Protein #42430

    Product Information

    Storage

    Human FGF-basic/FGF2 (147 aa) Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 16.5
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-basic.
    Activity The bioactivity of recombinant human FGF-basic was determined in a NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL.

    Source / Purification

    Recombinant human FGF-basic was expressed in E. coli and is supplied in a lyophilized form.

    Background

    FGF-basic (FGF2) is produced by epithelial, tumor, and other cell types (1). FGF-basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF-basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF-basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF-basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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