|Purity||A greater than or equal to 95% purity was determined by SDS-PAGE.|
|Endotoxin||Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-basic.|
|Activity||The bioactivity of recombinant human FGF-basic was determined in a NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL.|
Recombinant human FGF-basic was expressed in E. coli and is supplied in a lyophilized form.
FGF-basic (FGF2) is produced by epithelial, tumor, and other cell types (1). FGF-basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF-basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF-basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF-basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).
UniProt ID: P09038
Entrez-Gene Id: 2247
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