Working concentration of hFGF-basic generally ranges from 0.1-10 ng/ml.
|Purity||A greater than 95% purity was determined by SDS-PAGE.|
|Endotoxin||Less than or equal to 1 EU / 1 μg hFGF-basic|
|Activity||The bioactivity of Human FGF-basic Recombinant Protein was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05 - 1.0 ng/ml.|
Human FGF-basic Recombinant Protein was expressed in E. coli and is supplied in a lyophilized form.
FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
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