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95831
Human GDF15 Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human GDF15 Recombinant Protein #95831

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Human GDF15 Recombinant Protein: Image 1
The purity of Human GDF15 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hGDF15 and staining with Coomassie Blue. hGDF15 is a homodimer with a predicted total molecular weight (MW) of 24.8 kDa with each subunit equaling 12.4 kDa.

Storage

Human GDF15 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 24.8
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hGDF15.

Source / Purification

Recombinant human GDF15 was expressed in E. coli and is supplied in a lyophilized form.

Background

Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site, generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenesis, and metastasis, and may also contribute to weight loss during cancer (10,11).
  1. Strelau, J. et al. (2000) J Neurosci 20, 8597-603.
  2. Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6.
  3. Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4.
  4. Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7.
  5. Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92.
  6. Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9.
  7. Strelau, J. et al. (2000) J Neural Transm Suppl, 273-6.
  8. Kannan, K. et al. (2000) FEBS Lett 470, 77-82.
  9. Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9.
  10. Johnen, H. et al. (2007) Nat Med 13, 1333-40.
  11. Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 95831
Cat. # Size Qty. Price
95831S
20 µg
$ 258