Human GDNF Recombinant Protein #16187
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 30 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hGDNF. |
| Activity | The bioactivity of recombinant hGDNF was determined in a C6 cell proliferation assay. The ED50 of each lot is less than or equal to 3 μg/ml. |
Source / Purification
Recombinant human GDNF was expressed in E. coli and is supplied in a lyophilized form.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
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- Sariola, H. and Saarma, M. (2003) J Cell Sci 116, 3855-62.
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- Konishi, Y. et al. (2014) J Neurosci 34, 13127-38.
- Cintrón-Colón, A.F. et al. (2020) Cell Tissue Res 382, 47-56.
- Sharif, M. et al. (2021) Neurol Sci 42, 2865-2872.
- Choi, Y.D. et al. (2020) Int J Mol Sci 21, 3586. doi: 10.3390/ijms21103586.
- Yu, Z. et al. (2022) Int J Oncol 61, 109. doi: 10.3892/ijo.2022.5399.
Limited Uses
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