Human GM-CSF Recombinant Protein #87015
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 14.6 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hGM-CSF. |
| Activity | The bioactivity of recombinant hGM-CSF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 200 pg/mL. |
Source / Purification
Recombinant human GM-CSF was expressed in E. coli and is supplied in a lyophilized form.
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by activated T cells, NK cells, and macrophages (1,5). Target cells include granulocytes, monocyte precursors, and subsets of differentiated myeloid cells (1-3). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in hematopoietic differentiation of dendritic cells, and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (4). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5, and PI3K/Akt pathways (1).
Limited Uses
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