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Render Timestamp: 2024-10-11T10:24:54.092Z
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XML generation date: 2024-09-20 06:19:05.337
Product last modified at: 2024-07-18T18:00:08.242Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human Granulocyte Colony Stimulating Factor (hG-CSF) #8930

    Product Information

    Product Usage Information

    Reconstitution:

    With carrier: Add sterile 40 mM phosphate pH 4.0 to a final hG-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

    Carrier free: Add sterile 40 mM phosphate pH 4.0, or 40 mM phosphate pH 4.0 containing protein to minimize absorption of hG-CSF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hG-CSF should be greater than 50 μg/ml.

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl and 20 μg BSA per 1 μg hG-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl.

    Storage

    Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 18
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hG-CSF. All lots are greater than 98% pure.
    Endotoxin Less than 0.01 ng endotoxin/1μg hG-CSF.
    Activity The bioactivity of recombinant hG-CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 20-150 pg/ml.
    Molecular Formula Recombinant hG-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,986. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal TPLGP of recombinant hG-CSF was verified by amino acid sequencing.

    Source / Purification

    Recombinant human G-CSF (hG-CSF) Thr31-Pro204 (Accession #NP_757373) was expressed in human 293 cells at Cell Signaling Technology.

    Background

    G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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