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Product last modified at: 2024-09-27T22:30:09.072Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human IL-2 Recombinant Protein #31058

    Product Information

    Storage

    Human IL-2 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 15.5
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-2.
    Activity The bioactivity of recombinant hIL-2 was determined in a CTLL-2 cell proliferation assay. The ED50 of each lot is less than or equal to 3 ng/ml.

    Source / Purification

    Recombinant human IL-2 was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (4,5). Conversely, IL-2 is involved in activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (3). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2 Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15 (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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