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Human IL-5 Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human IL-5 Recombinant Protein #44227

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Human IL-5 Recombinant Protein: Image 1
The purity of Human IL-5 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hIL-5 and staining with Coomassie Blue. Human IL-5 is a homodimer with a predicted total molecular weight (MW) of 26.6 kDa with each subunit equaling 13.3 kDa.


Human IL-5 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Product Description

MW (kDa) 26
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-5.
Activity The bioactivity of recombinant hIL-5 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 250 pg/ml.

Source / Purification

Recombinant human IL-5 was expressed in E. coli and is supplied in a lyophilized form.


IL-5 is a pleiotropic cytokine that is predominantly produced by TH2 T cells but can also be expressed by activated eosinophils, mast cells, NK cells, and iNKT cells (1-4). Both human and mouse IL-5 are glycosylated disulfide-linked homodimers (1). The IL-5 receptor is a heterodimer that consists of a high affinity IL-5 binding α chain and the common β chain, which is shared by GM-CSF and IL-3 receptors, for signal transduction (1,2). Soluble IL-5Rα binds with high affinity to IL-5, thereby inhibiting IL-5 activity (1). IL-5-mediated signaling can activate the Erk1/2, Jak2, and Stat5 signaling pathways (1,2). In mice, IL-5 is important for the differentiation of antibody-secreting cells from activated B cells (2). IL-5 induces eosinophil activation, proliferation, and differentiation in both mice and humans (2,3).
  1. Takatsu, K. (2011) Proc Jpn Acad Ser B Phys Biol Sci 87, 463-85.
  2. Wang, W. et al. (2011) PLoS One 6, e17766.
  3. Kouro, T. and Takatsu, K. (2009) Int Immunol 21, 1303-9.
  4. Chuang, Y.H. et al. (2011) J Immunol 186, 4687-92.

Pathways & Proteins

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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 44227
Cat. # Size Qty. Price
10 µg
$ 215