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Render Timestamp: 2024-12-09T12:11:59.038Z
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XML generation date: 2024-12-06 01:01:08.118
Product last modified at: 2024-12-06T08:01:00.872Z
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Human Interferon-γ (hIFN-γ) Recombinant Protein #80385

    Product Information

    Product Usage Information

    Working concentration of hIFN-γ generally ranges from 0.1-10 ng/ml.

    Storage

    Recombinant human IFN-γ is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 17
    Purity A greater than 95% purity was determined by SDS-PAGE.
    Endotoxin Less than or equal to 1 EU / 1 μg hIFN-γ.
    Activity The bioactivity of hIFN-γ was determined in a virus protection assay. The ED50 of each lot is ≤ 0.75 ng/mL.

    Source / Purification

    Recombinant human IFN-γ was expressed in E. coli and is supplied in a lyophilized form.

    Background

    IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes the cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing expression of class I and class II MHC, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2 and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection in IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).
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