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XML generation date: 2026-01-28 15:11:38.035
Product last modified at: 2026-03-20T08:02:04.905Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Human Interleukin-1 alpha (hIL-1 alpha)

Human Interleukin-1 alpha (hIL-1 alpha) #8909

Inquiry Info. # 8909

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    Product Information

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

    Storage

    Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
    Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 22
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.
    Endotoxin Less than 0.01 ng endotoxin/1 μg hIL-1α.
    Activity The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 0.6-2.0 ng/ml.
    Molecular Formula Recombinant hIL-1α contains no "tags" and the nonglycosylated protein has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides due to glycosylation. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.

    Source / Purification

    Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #X03833) was expressed in human 293 cells at Cell Signaling Technology.

    Background

    IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NFκB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1α activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

    Alternate Names

    hIL-1; hIL-1alpha; IL-1; IL-1a; Interleukin 1alpha

    Database Links

    UniProt ID: P01583


    Entrez-Gene Id: 3552


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