Human Interleukin-3 (hIL-3) #8918
Product Information
Product Usage Information
Reconstitution:
With carrier: Add sterile PBS, or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hIL-3 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile PBS, or PBS containing protein to minimize absorption of hIL-3 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hIL-3 should be greater than 50 μg/ml.
Formulation
Storage
Product Description
| MW (kDa) | 26 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-3. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hIL-3. |
| Activity | The bioactivity of recombinant hIL-3 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50 -400 pg/ml. |
| Molecular Formula | Recombinant hIL-3 contains no "tags" and the nonglycosylated protein has a calculated MW of 15,091. DTT-reduced and non-reduced protein migrate as 26 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APMTQ of recombinant hIL-3 was verified by amino acid sequencing. |
Source / Purification
Recombinant human interleukin-3 (hIL-3) Ala20-Phe152 (Accession # NP_000579) was expressed in human 293 cells at Cell Signaling Technology.
Background
IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5).
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