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Human/Mouse/Rat Activin A Recombinant Protein #27313

    Product Information

    Storage

    Human/Mouse/Rat Activin A Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 26
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg h/m/r Activin A.
    Activity The bioactivity of recombinant h/m/r Activin A was determined in an MPC-11 cell cytotoxicity assay. The ED50 of each lot is less than or equal to 10 ng/ml.

    Source / Purification

    Recombinant human/mouse/rat Activin A was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Activin A is a pleiotropic cytokine that plays an important role in the regulation of the immune response and has been shown to possess both proinflammatory and anti-inflammatory activities (1-3). This cytokine is a member of the TGF-β superfamily known to signal through the Smad- and MAPK-dependent pathways by binding to the activin receptors type I (Alk2, Alk4) and type II (ActRIIA, ActRIIB) (1,2). Activin A is expressed by several cell types, including endothelial cells, mast cells, monocytes, macrophages, and dendritic cells (DCs) (4-6). The effect that Activin A has on the immune response makes it an important cytokine to study when looking at different diseases, such as allergy, autoimmunity, acute and chronic inflammation, and cancer (1,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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