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44909
Human TGF-α Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human TGF-α Recombinant Protein #44909

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Human TGF-α Recombinant Protein: Image 1
Serial dilutions of Human TGF-α Recombinant Protein were added to Balb/c-3T3 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
Human TGF-α Recombinant Protein: Image 2
The purity of Human TGF-α Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hTGF-α and staining with Coomassie Blue.

Storage

Human TGF-α Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile 10 mM HCl at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 5.6
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hTGF-α.
Activity The bioactivity of recombinant hTGF-α was determined in a Balb/c-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 2 ng/ml.

Source / Purification

Recombinant human TGF-α was expressed in Balb/c-3T3 cells and is supplied in a lyophilized form.

Background

Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differentiation (1). Members of the family share an EGF-like domain of 45-60 amino acids characterized by the conservation of six regularly spaced cysteines, forming three disulfide bonds that function as their receptor binding domain. TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and its name comes from its ability to induce transformation in cultured fibroblasts (2). This transforming activity was later shown to require TGF-beta, which potentiates the activity of TGF-alpha through a separate receptor (3). Soluble TGF-alpha is released from its membrane-bound precursor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4). Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components. TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5,6).
  1. Derynck, R. (1986) J Cell Biochem 32, 293-304.
  2. de Larco, J.E. and Todaro, G.J. (1978) Proc Natl Acad Sci U S A 75, 4001-5.
  3. Roberts, A.B. et al. (1981) Proc Natl Acad Sci U S A 78, 5339-43.
  4. Wong, S.T. et al. (1989) Cell 56, 495-506.
  5. Rusch, V. et al. (1996) Cytokine Growth Factor Rev 7, 133-41.
  6. Junier, M.P. (2000) Prog Neurobiol 62, 443-73.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 44909
Cat. # Size Qty. Price
44909S
25 µg
$ 95