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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human TGF-β1 Recombinant Protein #75362

    Product Information

    Storage

    Human TGF-β1 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile 10 mM HCl at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 25.6
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hTGF-β1.
    Activity The bioactivity of recombinant hTGF-β1 was determined in an IL-4 induced HT-2 cell proliferation assay. The ED50 of each lot is less than or equal to 0.5 ng/mL.

    Source / Purification

    Recombinant human TGF-β1 was expressed in CHO cells and is supplied in a lyophilized form.

    Background

    TGF-β1 activities include proliferation, angiogenesis, and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types, including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the SMAD proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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