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Human Transforming Growth Factor β2 (hTGF-β2)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Transforming Growth Factor β2 (hTGF-β2) #8406

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Citations (1)
Human Transforming Growth Factor β2 (hTGF-β2): Image 1
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hTGF-β2 was assessed. After 48 hour treatment with hTGF-β2, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.
Human Transforming Growth Factor β2 (hTGF-β2): Image 2
The purity of recombinant hTGF-β2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-β2 and staining overnight with Coomassie Blue.
Human Transforming Growth Factor β2 (hTGF-β2): Image 3
Western blot analysis of extracts from HT-1080 cells untreated or treated with TGF-β2 for 15 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).

Product Usage Information


With carrier: Add sterile 4 mM HCl containing 0.1% BSA to a final hTGF-β2 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile 4 mM HCl, or 4 mM HCl containing protein to minimize absorption of hTGF-β2 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hTGF-β2 should be greater than 50 μg/ml.


Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 12
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β2. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hTGF-β2.
Activity The bioactivity of recombinant hTGF-β2 was was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 0.1-0.3 ng/ml.
Molecular Formula Recombinant hTGF-β2 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,719. DTT-reduced protein migrates as a 12 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 24 kDa protein. The expected amino-terminal ALDAA of recombinant hTGF-β2 was verified by amino acid sequencing.

Source / Purification

Recombinant human TGF-β2 (hTGF-β2) Ala303-Ser414 (Accession #NP_003229) was expressed in human 293 cells at Cell Signaling Technology.


TGF-β2 is produced by eosinophils, astrocytes, glioblastoma and other cancer derived cell types (1-6). TGF-β2 inhibits antigen-induced T-cell proliferation, inhibits epithelial cell proliferation, induces mesenchymal cell proliferation and has cell specific effects on apoptosis (1-8). TGF-β2 binds to TβRII and binding is promoted by TβRIII. TβRI then complexes with TβRII and TβRIII (3,5). Signaling involves phosphorylation of the SMAD proteins (2,3,9). TGF-β2 also activates Erk2, p38, and JNK pathways (9). Knockout of TGF-β2 in mice severely impacts heart, lung and eye development (10).
  1. Balzar, S. et al. (2005) J Allergy Clin Immunol 115, 110-7.
  2. Siegel, P.M. and Massagué, J. (2003) Nat Rev Cancer 3, 807-21.
  3. Bierie, B. and Moses, H.L. (2006) Nat Rev Cancer 6, 506-20.
  4. Hinz, S. et al. (2007) Cancer Res 67, 8344-50.
  5. Damstrup, L. et al. (1993) Br J Cancer 67, 1015-21.
  6. Constam, D.B. et al. (1992) J Immunol 148, 1404-10.
  7. Zhang, H. et al. (2008) Immunology 124, 304-14.
  8. Dufour, C. et al. (2008) Am J Physiol Endocrinol Metab 294, E794-801.
  9. Moustakas, A. and Heldin, C.H. (2009) Development 136, 3699-714.
  10. Dünker, N. and Krieglstein, K. (2000) Eur J Biochem 267, 6982-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 8406
Cat. # Size Qty. Price
10 µg  (Carrier Free)
$ 238