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Human Transforming Growth Factor β3 (hTGF-β3)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Transforming Growth Factor β3 (hTGF-β3) #8425

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Human Transforming Growth Factor β3 (hTGF-β3): Image 1
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hTGF-β3 was assessed. After 48 hour treatment with hTGF-β3, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.
Human Transforming Growth Factor β3 (hTGF-β3): Image 2
The purity of recombinant hTGF-β3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-β3 and staining overnight with Coomassie Blue.
Human Transforming Growth Factor β3 (hTGF-β3): Image 3
Western blot analysis of extracts from HeLa cells untreated or treated with hTGF-β3 for 30 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).

Product Usage Information


With carrier: Add sterile 20 mM Citrate, pH 3.0 to a final hTGF-β3 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile 20 mM Citrate, pH 3.0, or 20 mM Citrate, pH 3.0 containing protein to minimize absorption of hTGF-β3 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hTGF-β3 should be greater than 50 μg/ml.


Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 12
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β3. Less than 5% migrates as monomer hTGF-β3 under non-reduced (-) conditions. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hTGF-β3.
Activity The bioactivity of recombinant hTGF-β3 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 0.02-0.1 ng/ml.
Molecular Formula Recombinant hTGF-β3 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,722. DTT-reduced protein migrates as a 12 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 22 kDa protein. The expected amino-terminal ALDTN of recombinant hTGF-β3 was verified by amino acid sequencing.

Source / Purification

Recombinant human TGF-β3 (hTGF-β3) Ala301-Ser412 (Accession #NP_003230) was expressed in human 293 cells at Cell Signaling Technology.


TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knock-out studies in mice, TGF-β3 is critical for lung and palate development (6).
  1. Siegel, P.M. and Massagué, J. (2003) Nat Rev Cancer 3, 807-21.
  2. Bierie, B. and Moses, H.L. (2006) Nat Rev Cancer 6, 506-20.
  3. Damstrup, L. et al. (1993) Br J Cancer 67, 1015-21.
  4. Constam, D.B. et al. (1992) J Immunol 148, 1404-10.
  5. Moustakas, A. and Heldin, C.H. (2009) Development 136, 3699-714.
  6. Dünker, N. and Krieglstein, K. (2000) Eur J Biochem 267, 6982-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 8425
Cat. # Size Qty. Price
10 µg  (With Carrier)
$ 238
10 µg  (Carrier Free)
$ 238