Working concentration of mIFN-γ generally ranges from 0.1-10 ng/ml.
Recombinant mouse IFN-γ is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
|Purity||A greater than 95% purity was determined by SDS-PAGE.|
|Endotoxin||Less than or equal to 1 EU / 1 μg mIFN-γ.|
|Activity||The bioactivity of mIFN-γ was determined in a virus protection assay. The biological activity of each lot is greater than or equal to 1.0 x 10^7 units/mg.|
Recombinant mouse IFN-γ was expressed in E. coli and is supplied in a lyophilized form.
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