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Mouse Interleukin-2 (mIL-2)

Mouse Interleukin-2 (mIL-2) #5201

Inquiry Info. # 5201

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Product Information

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM DTT and 20 μg BSA per 1 μg mIL-2. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM DTT.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa)	19
Purity	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-2. All lots are greater than 98% pure.
Endotoxin	Less than 0.01 ng endotoxin/1 μg mIL-2.
Activity	The bioactivity of recombinant mIL-2 was determined in a CTLL-2 cell proliferation assay. The ED₅₀ of each lot is between 0.5-2 ng/ml.
Molecular Formula	Based on amino acid sequencing, greater than 50% of recombinant mIL-2 has a Met on the amino-terminal Ala21 (MAPTS) and has a calculated MW of 17,231. The remainder starts at Ala21 (APTSS) or Pro22 (PTSSP). DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides.

Source / Purification

Recombinant mouse IL-2 (mIL-2) Ala21-Gln169 (Accession #NP_032392) was produced in E. coli at Cell Signaling Technology.

Background

IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (3,4). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15 (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

Alternate Names

il-2; il2; T cell growth factor; TCGF

Database Links

UniProt ID: P04351

Entrez-Gene Id: 16183

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