|64063||Mouse IL-7 Recombinant Protein||
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
|Purity||>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure.|
|Endotoxin||Less than 0.01 ng endotoxin/1 μg mIL-7.|
|Activity||The bioactivity of recombinant mIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 50-500 pg/ml.|
|Molecular Formula||Recombinant mIL-7 has a Met on the amino terminus and has a calculated MW of 15,028. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal MECHI of recombinant mIL-7 was verified by amino acid sequencing.|
Recombinant mouse IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397) was produced in E. coli at Cell Signaling Technology.
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