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5221
Human β-Nerve Growth Factor (hβ-NGF)

Human β-Nerve Growth Factor (hβ-NGF) #5221

Coomassie Gel Image 1

The purity of recombinant hβ-NGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hβ-NGF and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The proliferation of TF-1 cells treated with increasing concentrations of hβ-NGF was assessed. After 48 hour treatment with hβ-NGF, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.

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Western Blotting Image 3

Western blot analysis of extracts from TF-1 cells untreated or treated with hβ-NGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

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Recombinant human β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hβ-NGF. All lots are greater than 98% pure.

Molecular Formula:

Based on amino acid sequencing, greater than 85% of recombinant hβ-NGF starts at Ser122 (SSSHP) and has a calculated MW of 13,494. The remainder starts at Ser124 (SHPIF). DTT-reduced and non-reduced protein migrate as 13 kDa non-disulfide linked homodimers.

Bioactivity:

The bioactivity of recombinant hβ-NGF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 0.5-1.5 ng/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg hβ-NGF.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hβ-NGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

β-NGF is the prototypical member of the neurotrophin family of growth factors (1). β-NGF is involved in neuronal survival, differentiation and growth (1,2). Outside of the nervous system, NGF is produced by a variety of immune cells, including B cells, T cells, monocytes and mast cells (3,4). β-NGF binds to and signals through two distinct receptors, TrkA and p75NTR (1,2). Cellular responses induced by NGF are modulated by receptor expression. For example, TrKA leads to the inhibition of apoptosis and neuronal differentiation. In contrast, signaling through p75NTR in the absence of TrkA induces cell death. (1,2). NGF signaling via TrkA is characterized by activation of the PI3K/AKT and PLCγ pathways (1,2). NGF signaling via p75NTR induces JNK and NFκB activation (1,2). Aberrant NGF signaling may be linked to the onset of Alzheimer’s disease (5,6).

  1. Bibel, M. and Barde, Y.A. (2000) Genes Dev 14, 2919-37.
  2. Frossard, N. et al. (2004) Eur J Pharmacol 500, 453-65.
  3. Jiang, Y. et al. (2007) J Immunol 179, 6297-304.
  4. Matrone, C. et al. (2009) Proc Natl Acad Sci USA 106, 11358-63.
  5. Matrone, C. et al. (2008) Proc Natl Acad Sci USA 105, 13139-44.
  6. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
Entrez-Gene Id
4803
Swiss-Prot Acc.
P01138
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 5221SC

Product Number Size Price
5221SC 10 µg (With Carrier) $101.00.0
5221SF 10 µg (Carrier Free) $101.00.0
Quantity Subtotal
$0.00