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5221
Human β-Nerve Growth Factor (hβ-NGF)
Cytokines
Growth Factors and Cytokines

Human β-Nerve Growth Factor (hβ-NGF) #5221

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Citations (2)
Human β-Nerve Growth Factor (hβ-NGF): Image 1

The proliferation of TF-1 cells treated with increasing concentrations of hβ-NGF was assessed. After 48 hour treatment with hβ-NGF, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.

Human β-Nerve Growth Factor (hβ-NGF): Image 2

The purity of recombinant hβ-NGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hβ-NGF and staining overnight with Coomassie Blue.

Human β-Nerve Growth Factor (hβ-NGF): Image 3

Western blot analysis of extracts from TF-1 cells untreated or treated with hβ-NGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hβ-NGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hβ-NGF. All lots are greater than 98% pure.

Molecular Formula

Based on amino acid sequencing, greater than 85% of recombinant hβ-NGF starts at Ser122 (SSSHP) and has a calculated MW of 13,494. The remainder starts at Ser124 (SHPIF). DTT-reduced and non-reduced protein migrate as 13 kDa non-disulfide linked homodimers.

Source / Purification

Recombinant human β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497) was expressed in human 293 cells at Cell Signaling Technology.

Background

β-NGF is the prototypical member of the neurotrophin family of growth factors (1). β-NGF is involved in neuronal survival, differentiation and growth (1,2). Outside of the nervous system, NGF is produced by a variety of immune cells, including B cells, T cells, monocytes and mast cells (3,4). β-NGF binds to and signals through two distinct receptors, TrkA and p75NTR (1,2). Cellular responses induced by NGF are modulated by receptor expression. For example, TrKA leads to the inhibition of apoptosis and neuronal differentiation. In contrast, signaling through p75NTR in the absence of TrkA induces cell death. (1,2). NGF signaling via TrkA is characterized by activation of the PI3K/AKT and PLCγ pathways (1,2). NGF signaling via p75NTR induces JNK and NFκB activation (1,2). Aberrant NGF signaling may be linked to the onset of Alzheimer’s disease (5,6).

  1. Bibel, M. and Barde, Y.A. (2000) Genes Dev 14, 2919-37.
  2. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
  3. Frossard, N. et al. (2004) Eur J Pharmacol 500, 453-65.
  4. Jiang, Y. et al. (2007) J Immunol 179, 6297-304.
  5. Matrone, C. et al. (2009) Proc Natl Acad Sci USA 106, 11358-63.
  6. Matrone, C. et al. (2008) Proc Natl Acad Sci USA 105, 13139-44.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 5221SC
Product # Size Price
5221SC
10 µg  (With Carrier) $ 110
5221SF
10 µg  (Carrier Free) $ 110