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Human BMP-2
Growth Factors and Cytokines

Human BMP-2 #4697

Reviews ()
Citations (3)
other Image 1 - Human BMP-2

ATDC-5 cells were cultured with 0 to 1000 ng/mL of hBMP-2. Alkaline phosphatase activity was measured after 67 hours by measuring the OD405.

other Image 2 - Human BMP-2

The purity of recombinant hBMP-2 was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hBMP-2 and staining overnight with Coomassie Blue.

other Image 3 - Human BMP-2

Western blot analysis of extracts from serum-starved HT-1080 cells, untreated and treated for 30 minutes with various concentrations of Human BMP-2, using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 (upper) and Smad1 Antibody #9743 (lower).

Product Usage Information

The working concentration of BMP-2 generally ranges from 20-50 ng/ml.


Lyophilized product is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description




The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml.

Source / Purification

Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by reverse-phase HPLC and SDS-PAGE.


Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs are synthesized as precursor proteins that are processed by cleavage to produce mature proteins. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting in the translocation of Smad to the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).

  1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
  2. Wozney, J.M. et al. (1988) Science 242, 1528-34.
  3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
  4. Nohe, A. et al. (2004) Cell Signal 16, 291-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 4697S
Product # Size Price
10 µg $ 290