The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate, Z-Phe-Arg-AMC.
The purity of recombinant hCystatin C was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCystatin C and staining overnight with Coomassie Blue.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCystatin C. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCystatin C. All lots are greater than 98% pure.
Recombinant hCystatin C does not have a Met on the amino terminus and has a calculated MW of 13,347. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal SSPGK of recombinant hCystatin C was verified by amino acid sequencing.
The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate. The ED50 of each lot 1-12 nM.
Less than 0.01 ng endotoxin/1μg hCystatin C.
Recombinant human Cystatin C (hCystatin C) Ser27-Ala146 (Accession #NP_000090) was expressed in human 293 cells at Cell Signaling Technology.
Cystatin C is a 14 kDa member of the Cystatin superfamily of cysteine protease inhibitors (1). Most cell types secrete Cystatin C (1). Cystatin C inhibits cathepsins, and thereby may function as a tumor suppressor by inhibiting cathepsin mediated tumor cell invasion (2). In addition, this tumor suppressor function can also be attributed to Cystatin C's ability to antagonize TGF-β1 signaling (2,3). Cystatin C may also modulate antigen presentation through its ability to inhibit cathepsins (4). Cystatin C is also a biomarker for kidney dysfunction (5).
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