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8916
Human Epidermal Growth Factor (hEGF)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Epidermal Growth Factor (hEGF) #8916

Citations (6)

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# Product Name Applications Reactivity
The proliferation of MCF10A cells treated with increasing concentrations of hEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.
The purity of recombinant hEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hEGF and staining overnight with Coomassie Blue.
Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 6
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEGF. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hEGF.
Activity The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 10-600 pg/ml.
Molecular Formula Recombinant hEGF has a Met on the amino terminus and has a calculated MW of 6353. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSDS of recombinant hEGF was verified by amino acid sequencing.

Source / Purification

Recombinant human EGF (hEGF) Asn971-Arg1023 (Accession #NM_0011963) was produced in E. coli at Cell Signaling Technology.

Background

EGF is produced by epithelial cells, fibroblasts and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1,2, 3). There are multiple members of the EGF family and multiple members of the Erb/Her EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other Erb/Her members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and STAT 5 pathways (1). EGF, EGF family members, EGF receptors and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

Pathways

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Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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