Working concentration of hFGF-basic generally ranges from 0.1-10 ng/ml.
Human FGF-basic Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
|Purity||A greater than 95% purity was determined by SDS-PAGE.|
|Endotoxin||Less than or equal to 1 EU / 1 μg hFGF-basic|
|Activity||The bioactivity of Human FGF-basic Recombinant Protein was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05 - 1.0 ng/ml.|
Human FGF-basic Recombinant Protein was expressed in E. coli and is supplied in a lyophilized form.
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