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49812
Human FLT3L Recombinant Protein
Cytokines
Growth Factors and Cytokines

Human FLT3L Recombinant Protein #49812

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Human FLT3L Recombinant Protein: Image 1
Serial dilutions of Human FLT3L Recombinant Protein were added to OCI-AML5 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
Human FLT3L Recombinant Protein: Image 2
The purity of Human FLT3L Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hFLT3L and staining with Coomassie Blue.

Storage

Human FLT3L Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 18
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hFLT3L.
Activity The bioactivity of recombinant hFLT3L was determined in an OCI-AML5 cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/ml.

Source / Purification

Recombinant human FLT3L was expressed in E. coli and is supplied in a lyophilized form.

Background

FLT3L is produced by T cells and stromal fibroblasts (1,2). FLT3L targets many cell types, including hematopoietic stem cells, B cells, T cells, dendritic cells, and NK cells (1,2). FLT3L, in combination with other factors, stimulates differentiation and proliferation of hematopoietic multipotent progenitors and promotes proliferation of NK cells and dendritic cell subsets. There are two splice variants of FLT3L. Both the integral membrane and soluble splice variants are biologically active (1). Binding of FLT3L to its cognate tyrosine kinase receptor, FLT3, activates STAT3 and STAT5 (1,3). FLT3L is being tested for its ability to stimulate anti-tumor immune response via stimulation of dendritic and NK cells.
  1. Dong, J. et al. (2002) Cancer Biol Ther 1, 486-9.
  2. Wodnar-Filipowicz, A. (2003) News Physiol Sci 18, 247-51.
  3. Onai, N. et al. (2006) J Exp Med 203, 227-38.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 49812
Cat. # Size Qty. Price
49812S
10 µg $ 207