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50425
Human IL-15 Recombinant Protein
Cytokines
Growth Factors and Cytokines

Human IL-15 Recombinant Protein #50425

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Human IL-15 Recombinant Protein: Image 1
Serial dilutions of Human IL-15 Recombinant Protein were added to CTLL-2 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
Human IL-15 Recombinant Protein: Image 2
The purity of Human IL-15 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hIL-15 and staining with Coomassie Blue.

Storage

Human IL-15 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 12
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-15.
Activity The bioactivity of recombinant hIL-15 was determined in a CTLL-2 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/ml.

Source / Purification

Recombinant human IL-15 was expressed in E. coli and is supplied in a lyophilized form.

Background

Interleukin-15 (IL-15) is a pleiotropic cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). This glycoprotein is a member of the 4 α-helix-bundle family of cytokines and is required for the differentiation of effector CD8+ and memory phenotype CD8+ T cells (3,4). The effects of IL-5 are mediated through the heterotrimeric receptor consisting of the IL-15 receptor alpha (IL-15Rα), the β-chain, and the common γ-chain (2). The β-chain and common γ-chain are shared with the IL-2 receptor (3). Binding of IL-15 initiates signaling cascades that involve Jak1, Jak3, Stat3, and Stat5, leading to activation of the PI3K/Akt and Ras/Raf/MAPK signaling pathways (5). The effect that IL-15 has on innate and specific immunity makes it an important target for cancer immunotherapy and viral clearance (3,6).
  1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
  2. Perera, P.Y. et al. (2012) Microbes Infect 14, 247-61.
  3. Steel, J.C. et al. (2012) Trends Pharmacol Sci 33, 35-41.
  4. Perera, L.P. (2000) Arch Immunol Ther Exp (Warsz) 48, 457-64.
  5. Budagian, V. et al. (2006) Cytokine Growth Factor Rev 17, 259-80.
  6. Kandikattu, H.K. et al. (2020) Cytokine Growth Factor Rev, 54:24-31.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 50425
Cat. # Size Qty. Price
50425S
10 µg $ 207