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64210
Human IL-17A/F Heterodimer Recombinant Protein
Cytokines
Growth Factors and Cytokines

Human IL-17A/F Heterodimer Recombinant Protein #64210

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Human IL-17A/F Heterodimer Recombinant Protein: Image 1
The purity of Human IL-17A/F Heterodimer Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hIL-17A/F and staining with Coomassie Blue. hIL-17A/F is a heterodimer with a predicted total molecular weight (MW) of 30.7 kDa with the IL-17A monomer equaling 15.7 kDa and the IL-17F monomer equaling 15.0 kDa.

Storage

Human IL-17A/F Heterodimer Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 30.7
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-17A/F.
Activity The bioactivity of recombinant human IL-17A/F was determined by measuring the production of IL-6 from mouse 3T3 cells. The ED50 of each lot is less than or equal to 10 pg/mL.

Source / Purification

Recombinant human IL-17A/F was expressed in E. coli and is supplied in a lyophilized form.

Background

IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells and γδ T cells (1,2). IL-17A/F stimulates production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to activation of the Erk1/2 and NF-κB pathways (1).
  1. Iwakura, Y. et al. (2011) Immunity 34, 149-62.
  2. Chang, S.H. and Dong, C. (2007) Cell Res 17, 435-40.
  3. Henry, T. et al. (2010) J Immunol 184, 3755-67.
  4. Hu, Y. et al. (2010) J Immunol 184, 4307-16.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 64210
Cat. # Size Qty. Price
64210S
25 µg $ 257