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8846
Human Interleukin-17A/F Heterodimer (hIL-17A/F)
Cytokines
Growth Factors and Cytokines

Human Interleukin-17A/F Heterodimer (hIL-17A/F) #8846

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Human Interleukin-17A/F Heterodimer (hIL-17A/F): Image 1

The production of IL-6 by 3T3 MEFs WT cultured with increasing concentrations of hIL-17A/F was assessed. Media from cells incubated with hIL-17A/F for 24 hours was collected and assayed for IL-6 by ELISA and the OD450 was determined.

Human Interleukin-17A/F Heterodimer (hIL-17A/F): Image 2

The purity of recombinant hIL-17A/F was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-17A/F and staining overnight with Coomassie Blue.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17A/F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A/F. All lots are greater than 98% pure.

Molecular Formula

Based on amino acid sequencing, greater than 80% of recombinant hIL-17A/F starts at Gly24 (GITIP) and the remainder starts at Ile20 (IVKAG). The non-glycosylated heterodimer has a calculated MW of 31,396. The DTT-reduced and non-reduced protein migrate as 38 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.

Source / Purification

Recombinant human IL-17A/F (hIL-17A/F) is a heterodimer that is composed of hIL-17A Ile20-Ala155 (Accession# NP_002181) linked to hIL-17F Arg31-Glu163 (Accession# NP_443104) via the linker GGGSGGGSGGGSGGGS. hIL-17A/F was expressed in human 293 cells at Cell Signaling Technology.

Background

IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells, and γδ T cells (1,2). IL-17A/F stimulates the production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to the activation of the Erk1/2 and NF-κB pathways (1).

  1. Iwakura, Y. et al. (2011) Immunity 34, 149-62.
  2. Chang, S.H. and Dong, C. (2007) Cell Res 17, 435-40.
  3. Henry, T. et al. (2010) J Immunol 184, 3755-67.
  4. Hu, Y. et al. (2010) J Immunol 184, 4307-16.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 8846SC
Product # Size Price
8846SC
10 µg  (With Carrier) $ 221
8846SF
10 µg  (Carrier Free) $ 221