With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17A/F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
|Purity||>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A/F. All lots are greater than 98% pure.|
|Endotoxin||Less than 0.01 ng endotoxin/1 μg hIL-17A/F.|
|Activity||The bioactivity of recombinant hIL-17 A/F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 50-200 ng/ml.|
|Molecular Formula||Based on amino acid sequencing, greater than 80% of recombinant hIL-17A/F starts at Gly24 (GITIP) and the remainder starts at Ile20 (IVKAG). The non-glycosylated heterodimer has a calculated MW of 31,396. The DTT-reduced and non-reduced protein migrate as 38 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.|
Recombinant human IL-17A/F (hIL-17A/F) is a heterodimer that is composed of hIL-17A Ile20-Ala155 (Accession# NP_002181) linked to hIL-17F Arg31-Glu163 (Accession# NP_443104) via the linker GGGSGGGSGGGSGGGS. hIL-17A/F was expressed in human 293 cells at Cell Signaling Technology.
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