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- Analytical tools
Human Interleukin-17F (hIL-17F) #8906
This product is discontinued
The purity of recombinant hIL-17F was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-17F and staining overnight with Coomassie Blue.Learn more about how we get our images
Gallery: Human Interleukin-17F (hIL-17F) #8906
- The purity of recombinant hIL-17F was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-17F and staining overnight with Coomassie Blue.
- The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of hIL-17F was assessed. Media from cells incubated with hIL-17F for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.
Recombinant human IL-17F (hIL-17F) Arg31-Gln163 (Accession #NP_443104) was expressed in human 293 cells at Cell Signaling Technology.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17F. All lots are greater than 98% pure.Molecular Formula:
Recombinant hIL-17F contains no "tags" and the nonglycosylated protein has a calculated MW of 14,903. DTT-reduced protein migrates as a 22 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38 kDa protein. The expected amino-terminal RKIPK of recombinant hIL-17F was verified by amino acid sequencing.Bioactivity:
The bioactivity of recombinant hIL-17F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 30-80 ng/ml.Endotoxin:
Less than 0.01 ng endotoxin/1 μg hIL-17F.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
IL-17F is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell subset (1). IL-17F induces the production of pro-inflammatory cytokines, antimicrobial peptides, and neutrophil chemoattractants such as IL-6, β-defensin, IL-8, CXCL1, and CXCL6 (1,2). As a result, IL-17F may provide an important link between adaptive and the innate immunity. IL-17F binds with high affinity to IL-17RC, expressed primarily in non-hematopoietic tissues, and with lesser affinity to IL-17RA, which is expressed in hematopoietic tissues (3). IL-17F binding activates the ERK1/2 MAP kinase and NF-κB pathways (1,4). IL-17F appears to be involved in mucosal immunity against bacterial infections and possibly some autoimmune conditions (1,5,6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.