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Important Shipping Information

Due to a water main break, our U.S. warehouse is closed for the remainder of the day, Thursday, July 28. Orders placed today will ship tomorrow, Friday, July 29. We apologize for the inconvenience.

To Purchase # 8911SC

8911SC 10 µg (With Carrier) $199.00
8911SF 10 µg (Carrier Free) $199.00
8911LC 50 µg (With Carrier) $599.00
8911LF 50 µg (Carrier Free) $599.00
$0.00

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Source / Purification

Recombinant human LIF (hLIF) Ser23-Phe202 (Accession #P15018) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLIF. All lots are greater than 98% pure.


Molecular Formula:

Based on amino acid sequencing, greater than 30% of recombinant hLIF has a Met on the amino-terminal Ser23 (MSPLP) and has a calculated MW of 19,847. The remainder starts at Ser23 (SPLPI), Pro24 (PLPIT), or Pro26 (PITPV). DTT-reduced protein migrates as a 20 kDa polypeptide and non-reduced protein migrates as a 17 kDa polypeptide due to intramolecular cystines.


Bioactivity:

The bioactivity of recombinant hLIF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 0.2-1.5 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hLIF.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLIF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).


1.  Mathieu, M.E. et al. (2012) Stem Cell Rev 8, 1-15.

2.  Broholm, C. and Pedersen, B.K. (2010) Exerc Immunol Rev 16, 77-85.

3.  Cao, W. et al. (2011) Immunity 35, 273-84.

4.  Ullah, U. et al. (2012) Sci Rep 2, 464.


Entrez-Gene Id 3976
Swiss-Prot Acc. P15018


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

8911
Human Leukemia Inhibitory Factor (hLIF)