Serial dilutions of Human/Mouse/Rat Activin A Recombinant Protein were added to MPC-11 cells. Cell cytotoxicity was measured and the linear portion of the curve was used to calculate the ED50.
The purity of Human/Mouse/Rat Activin A Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant h/m/r Activin A and staining with Coomassie Blue. h/m/r Activin A is a homodimer with a predicted total molecular weight (MW) of 26.2 kDa with each subunit equaling 13.1 kDa.
Human/Mouse/Rat Activin A Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage
A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin levels are less than or equal to 1 EU / 1 μg h/m/r Activin A.
The bioactivity of recombinant h/m/r Activin A was determined in an MPC-11 cell cytotoxicity assay. The ED50 of each lot is less than or equal to 10 ng/ml.
Recombinant human/mouse/rat Activin A was expressed in E. coli and is supplied in a lyophilized form.
Activin A is a pleiotropic cytokine that plays an important role in the regulation of the immune response and has been shown to possess both proinflammatory and anti-inflammatory activities (1-3). This cytokine is a member of the TGF-β superfamily known to signal through the Smad- and MAPK-dependent pathways by binding to the activin receptors type I (Alk2, Alk4) and type II (ActRIIA, ActRIIB) (1,2). Activin A is expressed by several cell types, including endothelial cells, mast cells, monocytes, macrophages, and dendritic cells (DCs) (4-6). The effect that Activin A has on the immune response makes it an important cytokine to study when looking at different diseases, such as allergy, autoimmunity, acute and chronic inflammation, and cancer (1,5).
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