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3898
Human Neurotrophin-3 (NT-3)
Cytokines
Growth Factors and Cytokines

Human Neurotrophin-3 (NT-3) #3898

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# Product Name Applications Reactivity
Human Neurotrophin-3 (NT-3): Image 1
Western blot analysis of extracts from NIH/3T3 cells, transfected with TrkB and treated with 50 ng/ml and 100 ng/ml Human Neurotrophin-3 (NT-3) for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) and TrkB (80G2) Rabbit mAb (lower).

Product Usage Information

The working concentration for NT-3 generally ranges from 50-100 ng/ml.

Storage

Recombinant human NT-3 is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Product Description

Human NT-3 was prepared in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by HPLC and SDS-PAGE.
MW (kDa) 30
Purity >95%

Source / Purification

E. coli

Background

Neurotrophins are comprised of at least four family members including NGF, BDNF, NT-3 and NT-4 and all are known to influence growth, development, differentiation and survival of neurons (1). Proneurotrophins bind to p75NTR but not to the family of Trk receptor tyrosine kinases (Trk). Following maturation, NT-3 binds and activates TrkA, TrkB and TrkC, with TrkC almost exclusively binding to NT-3. Mature NT-4 binds to and activates TrkB (2). Trk receptors in turn activate three major signaling pathways: (a) Ras/MAPK, which promotes neuronal differentiation and neurite outgrowth, (b) PI3 Kinase/Akt, which promotes survival and growth of neurons, and (c) PLC-γ 1/PKC, which promotes synaptic plasticity (2).
NT-3 knock-out mice display movement and posture deficiencies due to proprioception defects as well as loss of neurons in spinal and cephalic sensory ganglia (3-4).
  1. Minichiello, L. and Klein, R. (1996) Genes Dev 10, 2849-58.
  2. Reichardt, L.F. (2006) Philos Trans R Soc Lond B Biol Sci 361, 1545-64.
  3. Tessarollo, L. et al. (1994) Proc Natl Acad Sci USA 91, 11844-8.
  4. Tessarollo, L. (1998) Cytokine Growth Factor Rev 9, 125-37.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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