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5367
Human Oncostatin M (hOSM)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Oncostatin M (hOSM) #5367

Citations (0)
The proliferation of TF-1 cells treated with increasing concentrations of hOSM was assessed. After 48 hour treatment with hOSM, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
The purity of recombinant hOSM was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hOSM and staining overnight with Coomassie Blue.
Western blot analysis of extracts from TF-1 cells, untreated or treated with hOSM for 10 minutes, using Phospho-Stat3 (Tyr705) (DA37) XP® Rabbit mAb #9145 (upper) and Stat3 Antibody #9132 (lower).

Product Usage Information

Reconstitution:

With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hOSM concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of hOSM to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hOSM should be greater than 50 μg/ml.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 32
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hOSM. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hOSM.
Activity The bioactivity of recombinant hOSM was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50-500 pg/ml.
Molecular Formula Based on amino acid sequencing, greater than 85% of recombinant hOSM starts at Aa26 (AAIGS) and has a calculated MW of 19,249. The remainder starts at Ile28 (IGSCS). DTT-reduced and non-reduced protein migrate as 32 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.

Source / Purification

Recombinant human Oncostatin M (hOSM) Ala26-Arg221 (Accession #NP_065391) was expressed in human 293 cells at Cell Signaling Technology.

Background

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. In contrast, Mouse OSM binds to the OSM receptor β (OSMRβ), which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). OSM induces activation of the Jak2, Stat3, and Erk1/2 pathways (5).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 5367
Cat. # Size Qty. Price
5367SF
10 µg  (Carrier Free)