Human Sonic Hedgehog (Shh) Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
|Purity||A greater than or equal to 95% purity was determined by SDS-PAGE.|
|Endotoxin||Endotoxin levels are less than or equal to 1 EU / 1 μg hShh.|
|Activity||The bioactivity of recombinant human Shh was determined in a CCL-226 cell proliferation assay. The ED50 of each lot is less than or equal to 5 μg/mL.|
Recombinant human Shh was expressed in E. coli and is supplied in a lyophilized form.
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