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9907
Human Stem Cell Factor (SCF)
Cytokines
Growth Factors and Cytokines

Human Stem Cell Factor (SCF) #9907

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# Product Name Application Reactivity
Human Stem Cell Factor (SCF): Image 1

Western blot analysis of extracts from MO7e cells, untreated or SCF-stimulated (50 ng/ml for 2 minutes), using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) or c-Kit Antibody #3392 (lower).

Product Usage Information

CST recommends using 50-100 ng/ml of SCF for stimulation of SCF signaling.

Storage

Human SCF is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeat freeze-thaw cycles.

Product Description

The human SCF coding cDNA was obtained from human bone marrow stromal cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human SCF protein was purified and stored in PBS buffer containing 0.1% BSA.

Purity

>97%

Source / Purification

Human Recombinant Protein

Background

Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).

  1. Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
  2. Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
  3. Huang, E. et al. (1990) Cell 63, 225-233.
  4. Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
  5. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
  6. Ueda, S. et al. (2002) Blood 99, 3342-3349.
  7. Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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