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5495
Human Transforming Growth Factor α (hTGF-α)
Cytokines
Growth Factors and Cytokines

Human Transforming Growth Factor α (hTGF-α) #5495

Citations (0)
Human Transforming Growth Factor α (hTGF-α): Image 1
The proliferation of MCF-10A cells treated with increasing concentrations of hTGF-α was assessed. After 24 hour treatment, cells were labeled with BrdU for 4 hours. BrdU incorporation was determined by ELISA and the OD450-OD650 was determined.
Human Transforming Growth Factor α (hTGF-α): Image 2
The purity of recombinant hTGF-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-α and staining overnight with Coomassie Blue.
Human Transforming Growth Factor α (hTGF-α): Image 3
Western blot analysis of extracts from HeLa cells untreated or treated with hTGF-α for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hTGF-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 5
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-α. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hTGF-α.
Activity The bioactivity of recombinant hTGF-α was determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 0.25-1.0 ng/ml.
Molecular Formula Based on amino acid sequencing, greater than 50% of recombinant hTGF-α has a Met on the amino-terminal Val40 (MVVSH) and has a calculated MW of 5,683. The remainder starts at Val40 (VVSHF). DTT-reduced and non-reduced protein migrate as 5 kDa polypeptides.

Source / Purification

Recombinant human TGF-α (hTGF-α) Val40-Ala89 (Accession #NP_003227) was produced in E. coli at Cell Signaling Technology.

Background

TGF-α is a member of the EGF family of proteins and is expressed by epithelial cells, keratinocytes, macrophages, and many tumor-derived cell lines (1-4). Soluble TGF-α is produced by proteolytic cleavage of a transmembrane precursor (5). TGF-α promotes cell proliferation and differentiation of a diverse array of cell types including cancer and neural cells (1,4,6). TGF-α binds to EGFR/ErbB1 and induces receptor dimerization and tyrosine kinase activation (7). TGF-α activates the MAPK pathways and involves Stat3 and Stat5 (1,6). Increased levels of TGF-α expression are associated with many cancers including breast cancer (1,8,9).
  1. Humphreys, R.C. and Hennighausen, L. (2000) Oncogene 19, 1085-91.
  2. Rappolee, D.A. et al. (1988) Science 241, 708-12.
  3. Xu, X. et al. (1999) Oncogene 18, 5554-62.
  4. Ciana, P. et al. (2003) J Biol Chem 278, 31737-44.
  5. Higashiyama, S. et al. (2008) Cancer Sci 99, 214-20.
  6. Song, J.I. and Grandis, J.R. (2000) Oncogene 19, 2489-95.
  7. Citri, A. and Yarden, Y. (2006) Nat Rev Mol Cell Biol 7, 505-16.
  8. Révillion, F. et al. (2008) Ann Oncol 19, 73-80.
  9. Masago, K. et al. (2008) Cancer Sci 99, 2295-301.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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