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9954
Interleukin-6 (IL-6)
Cytokines
Growth Factors and Cytokines

Interleukin-6 (IL-6) #9954

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# Product Name Application Reactivity
Interleukin-6 (IL-6): Image 1

Western blot analysis of extracts from human myeloma cells stimulated with rhIL-6 (100 ng/ml for 10 min.), using Phospho-Stat1(Tyr701) Antibody #9171 (upper A), Stat1 Antibody #9172 (lower A), Phospho-Stat3(Tyr705) Antibody #9131 (upper B), Stat3 Antibody #9132 (lower B), Phospho-Jak1(Tyr1022/1023) Antibody #3331 (upper C) and Jak1 Antibody #3332 (lower C).

Product Usage Information

CST recommends using 50-100 ng/ml for stimulation of IL-6 signaling.

Storage

Human IL-6 is supplied in PBS buffer containing 0.1% BSA. Aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. F

Product Description

Human IL-6 cDNA was subcloned into a prokaryotic expression vector, expressed in E. coli, and recombinant human Interleukin-6 (rhIL-6) protein was purified.

Purity

>98%

Source / Purification

Human Recombinant Protein

Background

Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6-family members such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Ralpha , 80 kDa) and two beta chains (IL-6Rbeta or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways : JAK/STAT, PI-3 kinase and MAPK signaling pathways (2,3).

  1. Ito, H. (2003) Curr Drug Targets Inflamm Allergy 2, 125-30.
  2. Taniguchi, T. (1995) Science 268, 251-5.
  3. Boulton, T.G. et al. (1994) J Biol Chem 269, 11648-55.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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