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5331
Mouse Epidermal Growth Factor (mEGF)
Cytokines & Growth Factors
Growth Factors and Cytokines

Mouse Epidermal Growth Factor (mEGF) #5331

Citations (0)
The proliferation of NIH/3T3 cells treated with increasing concentrations of mEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined using the BrdU Cell Proliferation Assay Kit #6813 and the OD450 was determined.
The purity of recombinant mEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mEGF and staining overnight with Coomassie Blue.
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with mEGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Product Usage Information

Reconstitution:

With carrier: Add sterile 20 mM citrate, pH 3.0 to a final mEGF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile 20 mM citrate, pH 3.0 or 20 mM citrate, pH 3.0 containing protein to minimize absorption of mEGF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mEGF should be greater than 50 μg/ml.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 6
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mEGF. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg mEGF.
Activity The bioactivity of recombinant mEGF was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 10-250 pg/ml.
Molecular Formula Recombinant mEGF has a Met on the amino terminus and has a calculated MW of 6045. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSYP of recombinant mEGF was verified by amino acid sequencing.

Source / Purification

Recombinant mouse EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) was produced in E. coli at Cell Signaling Technology.

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 5331
Cat. # Size Qty. Price
5331SC
50 µg  (With Carrier)
5331SF
50 µg  (Carrier Free)