Buy 3 Get a 4th Free* | Learn More >>
11996
Mouse His6Interleukin-25/IL-17E (mHis6IL-25)

Mouse His6Interleukin-25/IL-17E (mHis6IL-25) #11996

This product is discontinued

Coomassie Gel Image 1

The purity of recombinant mHis6IL-25 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant mHis6IL-25 and staining overnight with Coomassie Blue.

Learn more about how we get our images
Bioactivity Image 2

IL-13 production from mouse splenocytes cultured with mHis6IL-25 was assessed. Mouse splenoyctes were treated with increasing concentrations of mHis6IL-25 for 72 hr. Cell supernatants were then harvested and assayed for IL-13 by ELISA, and the OD450 was determined.

Learn more about how we get our images
Bioactivity Image 3

GRO α production from HT-29 cells cultured with mHis6IL-25 was assessed. HT-29 cells were treated with increasing concentrations of mHis6IL-25 for 24 hr. Cell supernatants were then harvested and assayed for GRO α by ELISA, and the OD450 was determined.

Learn more about how we get our images

Recombinant mouse His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-25. All lots are greater than 97% pure.

Molecular Formula:

Recombinant N-terminally His6-tagged mIL-25 has a calculated MW of 20,051 Da. DTT-reduced protein migrates as a 24-31 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 46-50 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant mHis6IL-25 was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant mHis6IL-25 was determined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5 ng/ml in mouse splenocyte cells and 0.25-1.5 ng/mL in HT-29 cells.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg mHis6IL-25.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).

  1. Iwakura, Y. et al. (2011) Immunity 34, 149-62.
  2. Rickel, E.A. et al. (2008) J Immunol 181, 4299-310.
  3. Petersen, B.C. et al. (2012) Nat Med 18, 751-8.
  4. Angkasekwinai, P. et al. (2010) Nat Immunol 11, 250-6.
  5. Furuta, S. et al. (2011) Sci Transl Med 3, 78ra31.
Entrez-Gene Id
140806
Swiss-Prot Acc.
Q8VHH8
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.