The purity of Mouse IL-22 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mIL-22 and staining with Coomassie Blue.
Mouse IL-22 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-22.
The bioactivity of recombinant mIL-22 was determined by measuring the production of IL-10 from human Colo 205 cells. The ED50 of each lot is less than or equal to 1 ng/ml.
Recombinant mouse IL-22 was expressed in E. coli and is supplied in a lyophilized form.
Interleukin-22 (IL-22), a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells, and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells (4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue repair and wound healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6).
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