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5371
Mouse Oncostatin M (mOSM)
Cytokines
Growth Factors and Cytokines

Mouse Oncostatin M (mOSM) #5371

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Mouse Oncostatin M (mOSM): Image 1
The proliferation of NIH/3T3 cells treated with increasing concentrations of mOSM was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.
Mouse Oncostatin M (mOSM): Image 2
The purity of recombinant mOSM was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mOSM and staining overnight with Coomassie Blue.
Mouse Oncostatin M (mOSM): Image 3
Western blot analysis of extracts from MEF cells untreated or treated with mOSM for 15 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mOSM. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 22
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mOSM. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg mOSM.
Activity The bioactivity of recombinant mOSM was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.10-0.40 ng/ml.
Molecular Formula Recombinant mOSM does not have Met on the amino terminus and has a calculated MW of 20,750. DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides. The expected amino-terminal ANRGC of recombinant mOSM was verified by amino acid sequencing.

Source / Purification

Recombinant mouse OSM (mOSM) Ala24-Arg206 (Accession #NP_001013383) was produced in E. coli at Cell Signaling Technology.

Background

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). In contrast, human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. OSM induces the activation the JAK2, STAT3, and ERK1/2 pathways (5).
  1. Malik, N. et al. (1989) Mol Cell Biol 9, 2847-53.
  2. Silver, J.S. and Hunter, C.A. (2010) J Leukoc Biol 88, 1145-56.
  3. Underhill-Day, N. and Heath, J.K. (2006) Cancer Res 66, 10891-901.
  4. Ichihara, M. et al. (1997) Blood 90, 165-73.
  5. Hintzen, C. et al. (2008) J Immunol 181, 7341-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 5371SC
Product # Size Price
5371SC
25 µg  (With Carrier) $ 227
5371SF
25 µg  (Carrier Free) $ 227