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68495
Mouse RANKL/TRANCE/TNFSF11 Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Mouse RANKL/TRANCE/TNFSF11 Recombinant Protein #68495

Citations (0)
Serial dilutions of Mouse RANKL/TRANCE/TNFSF11 Recombinant Protein were added to RAW-Blue cells. The induction of secreted embryonic alkaline phosphatase (SEAP) production was measured and the linear portion of the curve was used to calculate the ED50.
The purity of Mouse RANKL/TRANCE/TNFSF11 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mRANKL and staining with Coomassie Blue.

Storage

Mouse RANKL/TRANCE/TNFSF11 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 19
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg mRANKL.
Activity The bioactivity of recombinant mRANKL was determined in a secreted embryonic alkaline phosphatase (SEAP) induction assay using RAW-Blue™ cells. The ED50 of each lot is less than or equal to 50 ng/ml. RAW-Blue™ Cells were purchased from InvivoGen Inc. 

Source / Purification

Recombinant mouse RANKL was expressed in E. coli and is supplied in a lyophilized form. This product was produced without any FBS or animal-derived ingredients.

Background

RANKL, also known as TRANCE, TNFSF11, or OPGL, is a member of the TNF superfamily of ligands. T cells, mammary epithelial cells, and endothelial cells can produce RANKL (1). RANKL is expressed as a type II transmembrane protein or cleaved into a soluble form by extracellular proteases, such as TACE, ADAM10, and matrix metalloproteases (1). Alternative splicing also results in the production of soluble RANKL (1). RANKL signaling is antagonized by osteoprotegerin, which functions as a soluble decoy receptor (2). RANKL plays key roles in mammary gland development and dendritic cell survival and is required for osteoclast differentiation and survival (3-6). Research studies have shown that RANKL deficiencies in both mice and humans are associated with abnormally increased bone density and defects in lymphoid organogenesis (5,6).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 68495
Cat. # Size Qty. Price
68495S
10 µg