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5211
Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )
Cytokines & Growth Factors
Growth Factors and Cytokines

Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 ) #5211

Citations (0)
The proliferation of HUVEC treated with increasing concentrations of mVEGF164 was assessed. After 72-hour treatment with mVEGF164 cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.
The purity of recombinant mVEGF164 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mVEGF164 and staining overnight with Coomassie Blue.
Western blot analysis of extracts from HUVEC untreated or treated with mVEGF164 for 15 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Product Usage Information

Reconstitution:

With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final mVEGF164 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of mVEGF164 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mVEGF164 should be greater than 50 μg/ml.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 24-31
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF164. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1μg mVEGF164.
Activity The bioactivity of recombinant mVEGF164 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.
Molecular Formula Recombinant mVEGF164 contains no "tags" and the nonglycosylated protein has a calculated MW of 19,278. DTT-reduced protein migrates as a 24-31 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38-44 kDa protein. The expected amino-terminal APTTE of recombinant mVEGF164 was verified by amino acid sequencing.

Source / Purification

Recombinant mouse VEGF164 (mVEGF164) Ala205-Arg368 (Accession #NP_033531) was expressed in human 293 cells at Cell Signaling Technology.

Background

VEGF164 is one of many splice variants of the mouse VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1,2). VEGF164 is produced by a number of cells including endothelial cells, macrophages and T cells (1,2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and Paxillin (2).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 5211
Cat. # Size Qty. Price
5211SC
10 µg  (With Carrier)
5211SF
10 µg  (Carrier Free)