Interested in promotions? | Click here >>
9943
Vascular Endothelial Growth Factor (VEGF)
Cytokines
Growth Factors and Cytokines

Vascular Endothelial Growth Factor (VEGF) #9943

Citations (0)

We recommend the following alternatives

# Product Name Application Reactivity
Vascular Endothelial Growth Factor (VEGF): Image 1

Western blot analysis of extracts from HUVE cells, untreated or VEGF165 stimulated (100 ng/ml for 5 min), using Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (upper) or VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower).

Product Usage Information

CST recommends using 50-100 ng/ml of VEGF for stimulation of VEGF signaling.

Storage

Vascular Endothelial Growth Factor (VEGF) is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeat freeze-thaw cycles.

Product Description

The human VEGF165 coding cDNA was subcloned into an expression vector and expressed in yeast. The recombinant human VEGF 165 homodimer was purified and stored in PBS buffer (pH 7.4) containing 0.1% BSA.

Purity

>95%

Source / Purification

Human Recombinant Protein

Background

VEGF is a heparin-binding glycoprotein that is secreted as a homodimer of 45 kDa. Five human VEGF mRNA species encoding VEGF isoforms of 121, 145, 165, 189, and 206 amino acids are produced by alternative splicing of the VEGF mRNA from a single gene (1). Transcripts encoding VEGF165 and VEGF121 are detected in the majority of cells and tissues expressing the VEGF gene. VEGF121 lacks the amino acids encoded by exon 7 of the VEGF gene, which is present in VEGF165 and enables the ability of VEGF165 to bind to heparin and heparan sulfate. Heparin-binding VEGF165 is the best characterized VEGF species. The binding of VEGF165 to the VEGF receptors of vascular endothelial cells is modulated by the addition of exogenous heparin or heparan sulfate and inhibited after digestion of endothelial cells with heparinase (2). Neuropilin-1 is also a VEGF165 isoform specific receptor. It has been reported that neuropilin-1 can enhance the binding of VEGF165 to the Flk-1/KDR receptor (3). VEGF165 expression is regulated by various stimuli including growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations.

  1. Houck, K. A. et al. (1991) Mol Endocrinol 5, 1806-1814.
  2. Gitay-Goren, H. et al. (1992) J. Biol. Chem. 267, 6093-6098.
  3. Soker, S. et al. (1998) Cell 92, 735-745.
  4. Loureiro, R.M. and D'Amore, P.A. (2005) Cytokine Growth Factor Rev. 16, 104-113.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9943