Figure 2: The relationship between protein concentration of lysates from untreated and TSA treated COS cells and kit assay optical density readings. COS cells were treated with TSA (4 µM overnight). An acid extraction was performed for cell lysis in the presence of 5 mM sodium butyrate.
CST's PathScan® Acetyl-Histone H2A Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetylated lysines on Histone H2A. A Histone H2A Antibody has been coated onto the microwells. After incubation with cell lysates, Histone H2A is captured by the coated antibody. Following extensive washing, Acetylated-Lysine Rabbit mAb (Ac-K2-100) is added to detect the acetylated lysines on the Histone H2A protein. Anti-rabbit IgG, HRP linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of acetylated Histone H2A.
Antibodies in kit are custom formulations specific to kit.
CST's PathScan® Acetyl-Histone H2A Sandwich ELISA Kit detects endogenous levels of Acetylated Histone H2A. Using this Sandwich ELISA Kit #7233, acetylated lysines on Histone H2A are detected when treated with TSA in Jurkat cells. However, the levels of Histone H2A remain unchanged, as shown by Western analysis using the Histone H2A Antibody #2578 (figure 1). COS and NIH 3T3 cells treated with TSA show similar results (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.