View Featured Offers >>
42173
FastScan™ Phospho-p44/42 MAPK (Thr202/Tyr204) ELISA Kit
ELISA Kits
FastScan ELISA Kit

FastScan Phospho-p44/42 MAPK (Thr202/Tyr204) ELISA Kit #42173

Citations (0)
Figure 1. Treatment of A-431 cells with EGF stimulates phosphorylation of p44/42 MAPK (Erk1/2) at Thr202 and Tyr204 but does not effect the level of total p44/42 MAPK (Erk1/2). The relationship between lysate protein concentration from untreated and EGF-treated A-431 cells and the absorbance at 450 nm using the FastScan Phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) ELISA Kit #42173 is shown in the upper figure. The corresponding western blots using phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (left panel) and p44/42 MAPK (Erk1/2) antibody (right panel) are shown in the lower figure. After serum starvation, A-431 cells were treated with 100 ng/ml EGF #8916 for 5 minutes at 37°C and then lysed.

Product Description

The FastScan Phospho-p44/42 MAPK (Thr202/Tyr204) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of p44/42 MAPK when phosphorylated at Thr202/Tyr204. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-p44/42 MAPK (Thr202/Tyr204) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-p44/42 MAPK (Thr202/Tyr204).

*Antibodies in this kit are custom formulations specific to kit.

Storage

Kit should be stored at 4°C.

Specificity / Sensitivity

The FastScan Phospho-p44/42 MAPK (Thr202/Tyr204) ELISA Kit detects endogenous levels of p44/42 MAPK when phosphorylated at Thr202/Tyr204, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

Pathways

Explore pathways related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
To Purchase # 42173
Cat. # Size Qty. Price
42173C
1 Kit  (96 assays)

Important Ordering Details

Custom Ordering Details:

When ordering five or more kits, please contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.