Cat. # | Size | Qty. | Price |
---|---|---|---|
88197C | 1 Kit (96 assays) |
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When ordering five or more kits, please contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
REACTIVITY | All |
Product Includes | Volume (with Count) | Solution Color | |||
---|---|---|---|---|---|
FastScan™ ELISA Microwell Strip Plate, 96 Well 53257 | 1 x 96 tests | ||||
LbCpf1 (Strain ND2006)/Cas12a Mouse Capture mAb | 1 x 1 ea | Green (Lyophilized) | |||
LbCpf1 (Strain ND2006)/Cas12a Rabbit HRP-linked mAb | 1 x 1 ea | Red (Lyophilized) | |||
FastScan™ ELISA Capture Antibody Diluent | 1 x 3 ml | Green | |||
FastScan™ ELISA HRP Antibody Diluent | 1 x 3 ml | ||||
TMB Substrate 7004 | 1 x 11 ml | ||||
STOP Solution 7002 | 1 x 11 ml | ||||
Sealing Tape | 1 x 1 ea | ||||
ELISA Wash Buffer (20X) 9801 | 1 x 25 ml | ||||
FastScan™ ELISA Cell Extraction Buffer (5X) 69905 | 1 x 10 ml | ||||
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) 25243 | 1 x 1 ml | ||||
FastScan™ ELISA Kit #88197 Positive Control Type 2 | 1 x 1 ea |
Product Information
NOTE: Prepare solutions with deionized/purified water or equivalent.
Prepare only as much reagent as needed on the day of the experiment.
*IMPORTANT: The provided FastScan™ ELISA Cell Extraction Enhancer Solution (50X) may precipitate when stored at 4°C. To dissolve, warm briefly at 37°C and mix gently. The FastScan™ ELISA Cell Extraction Enhancer Solution (50X) can be stored at room temperature to avoid precipitation.
NOTE: The 1X Cell Extraction Buffer contains phosphatase inhibitors. Protease inhibitors should be added to the 1X Cell Extraction Buffer immediately prior to lysing cells. Additional phosphatase inhibitors can also be added (e.g. Protease/Phosphatase Inhibitor Cocktail (100X) #5872, not supplied).
NOTE: A select number of FastScan™ ELISA kits do not contain a positive control, please refer to Product Includes table on the datasheet for a list of included reagents. Should you need support on how to generate a positive control for those kits, contact CST technical support at [email protected].
For adherent cells
For suspension cells
NOTE: Equilibrate all materials and prepared reagents to room temperature prior to running the assay.
*NOTE: Certain FastScan™ ELISA Kits may require additional washes at this step. Any requirements for additional washes will be specifically noted in the product “Description” of the kit’s datasheet.
NOTE: Initial color of positive reaction is blue, which changes to yellow upon addition of STOP Solution.
posted May 2018
revised November 2019
Protocol Id: 1924
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).~LbCpf1 (Strain ND2006)/Cas12a is a Cpf1/Cas12a enzyme derived from Lachnospiraceae bacterium ND2006 (2,4).
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